dmem f12 medium (ATCC)

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ATCC dmem f12 medium
Dmem F12 Medium, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 5689 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dmem+f12+medium/DMEM%3A+F-12+Medium/pm42898112-259-20-12
Average 99 stars, based on 5689 article reviews

dmem f12 medium - by Bioz Stars, 2026-10

99/100 stars

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Cell Culture:

Article Title: Extracellular vesicle-mediated preconditioning of osteoblasts by ER+ breast cancer cells: implications for the bone metastatic niche
Article Snippet: Medium was also supplemented with 10% FBS (ATCC, 30–2020), and human recombinant insulin, zinc solution (Gibco; 12-585-014), at a final concentration of 0.2 U/mL (T47D media). .. Human MCF10A breast epithelial cells (ATCC, CRL-10317) were cultured in DMEM/F12 medium (ATCC, 30–2006) supplemented with 5% horse serum (ATCC, 30–2030), human recombinant epidermal growth factor (EGF; Gibco, PHG0311) at a final concentration of 20 ng/mL, hydrocortisone (Sigma-Aldrich, H0888) at 0.5 μg/mL, cholera toxin (Sigma-Aldrich, C8052) at 100 ng/mL, and human recombinant insulin (Gibco, 12-585-014) at 10 μg/mL. ..

Article Title: Dual impact of EZH1/2-targeted therapy in HTLV-1-associated inflammation: Direct and indirect mechanisms.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Ponceau S Staining Solution Thermo Fisher Scientific Cat# A40000279 2-Mercaptoethanol Wako Cat# 13575-45 Laemmli buffer Bio-Rad Cat# 1610737 Normal Goat Serum Thermo Fisher Scientific Cat# 31872 TE buffer (PH = 8.0) FUJIFILM Cat# 314-90021 Critical commercial assays Annexin V-FITC/PI (propidium iodide) staining kit Abcam Cat# ab14085 CBA Human Inflammatory Cytokine kit BD, Biosciences Cat# 551811; RRID: AB_2868941 CBA Human Chemokine kit BD, Biosciences Cat# 552990; RRID: AB_2868970 Human IL-8 ELISA Kit Thermo Fisher Scientific Cat# KAC1301 Human IL-6 ELISA Kit Thermo Fisher Scientific Cat# EH2IL6 EZ1 Virus Mini Kit v2.0 QIAGEN Cat# 955134 CCK-8 assay AbMole Bioscience Cat# M4839 NF-κB InstantOne ELISA Kit eBioscience Cat# 85-86083-11 Protein concentration detection: BCA assay kit Thermo Fisher Scientific Cat# 23227 RNeasy Kits for RNA Purification Qiagen Cat# 74104 SuperScriptTM III PlatinumTM SYBRTM Green One-Step qRT-PCR Kit Thermo Fisher Scientific Cat# 11736051 Experimental models: Cell lines Human cell line: ARPE-19 ATCC Cat# CRL-2302; RRID: CVCL_0145 Primary human cells: hTMCs ScienCell Research Laboratories Cat# 6590 Human cell line: MT-2 JCRB Cell Bank (Osaka, Japan) JCRB1210; RRID: CVCL_2631 Human cell line: TL-Om1 The Institute of Medical Science, The University of Tokyo RRID: CVCL_B473 Human cell line: Jurkat ATCC Cat# TIB-152; RRID: CVCL_0367 Oligonucleotides RT-qPCR primers: EZH1-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAATTCAAGCTGGCGAAGAG-3′ RT-qPCR primers: EZH1-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAAGACAGTGCCGCTACCA-3′ RT-qPCR primers: EZH2-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GCCAAGAGAGCCATCCAGAC-3′ RT-qPCR primers: EZH2-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCGACATACTTCAGGGCATCA-3′ RT-qPCR primers: Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- TCAACCCTCACCACTCCAGGC-3′ RT-qPCR primers: Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGGGCTCATGGTCATTGTCAT-3′ RT-qPCR primers: HBZ-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-AAGAGCATCGACGACATCGG-3′ RT-qPCR primers: HBZ-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- GTGGGACAGGAGCTTGAAGT-3′ Proviral load primers: HTLV-1 Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCCACTTCCCAGGGTTTGGA-3′ Proviral load primers: HTLV-1 Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGCCAGTAGGGCGTGA-3′ Proviral load primers: probe This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-FAM-CCAGTCTACGTGTTTG GAGACTGTGTACA-TAMRA-3′ (Continued on next page) 18 iScience 29, 117775, November 20, 2026 .. Cell lines and culture conditions ARPE-19 cells (CRL-2302) were obtained from the American Type Culture Collection (ATCC) and cultured in DMEM: F12 medium supplemented with 10% heat-inactivated fetal bovine serum and 1% penicillin-streptomycin solution. ..

Article Title: The LINC00920/miR-6834-3p/CPN1 Axis Modulates Immune Dysregulation in Sepsis-Associated Acute Kidney Injury and Serves as a Diagnostic Biomarker.
Article Snippet: Sepsis-associated acute kidney injury (SA-AKI) is a serious complication with high mortality and lacks effective diagnostic and treatment strategies.. This study aims to investigate the role of the LINC00920/miR-6834-3p/CPN1 axis in SA-AKI and evaluate its diagnostic value.. Clinical serum samples and in vitro cell models were used to explore the role of the LINC00920/miR-6834-3p/CPN1 axis in SA-AKI.

Article Title: Structural insights into single-pass transmembrane receptor GC-A activation by distinct antihypertensive antibodies.
Article Snippet: .. HEK293 cells (ATCC CRL-1573) were cultured in DMEM/F12 medium containing 10% fetal calf serum and AR TI CL E IN P RE SS transfected with wild-type full-length human GC-A. .. HEK293 cells (ATCC CRL-1573) were cultured in DMEM/F12 medium containing 10% fetal calf serum and AR TI CL E IN P RE SS transfected with wild-type full-length human GC-A.

Article Title: Lesions initiating spontaneous mitotic crossover are minimally subject to non-homologous end joining
Article Snippet: .. BJ-5ta (CRL-4001, ATCC) cells were cultured in DMEM/F12 medium supplemented with 10% FBS and 1x Pen-Strep. ..

Article Title: The dual IGF-1R/IR blocker, Linsitinib, modulates human orbital fibroblast activity and proliferation
Article Snippet: .. The IGF-1R-expressing adrenocortical cell line NCI-H295R (ATCC, Manassas, VA, USA) was cultured in DMEM: F12 medium (ATCC) supplemented with 2.5% Nu-Serum (Corning, Corning, NY, USA) and ITS+ Premix (Corning) containing insulin, transferrin, selenium, bovine serum albumin, and linoleic acid. ..

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Article Title: MicroRNA-132 Alleviates Ischemia-Reperfusion-Induced Neuronal Apoptosis by Targeting Mecp2 in Stroke Models.
Article Snippet: The human neuroblastoma SH-SY5Y cell line (ATCC® CRL-2266TM) was maintained in 1:1 DMEM/F12 medium supplemented with 10% fetal bovine serum (Gibco, USA), 100 U/mL penicillin, and 100 μg/mL streptomycin in a humidified 5% CO2 incubator at 37 °C.

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